Servisní laboratoř průtokové cytometrie

FACSymphony (BD Biosciences) flow cytometer is equipped with six solid state lasers (355, 405, 447, 488, 561 and 637nm). It has 31 fluorescent detectors and two additional detectors for forward and right angle light scattering. Default optical configuration of the instrument enables the measurement of practically all frequently used fluorochromes and fluorescent proteins. Red sensitive GaAsP detectors are used for detection in all far red channels (over 700 nm). It features low noise VPX electronics. Instrument is equipped with High Throughput Sampler for high-throughput measurement of samples directly from 96- or 384-well plates.

LSRII (BD Biosciences) flow cytometer is equipped with four solid state lasers (405, 488, 561 and 638nm) and 18 fluorescent detectors and two additional detectors for forward and right angle light scattering. Default optical configuration of the instrument enables the measurement of many frequently used fluorochromes and fluorescent proteins. Large set of dichroic mirrors and bandpass filters is available in the laboratory, making this instrument customizable and capable to cover many different flow cytometry applications. Instrument is equipped with High Throughput Sampler for high-throughput measurement of samples directly from 96- or 384-well plates.

Amnis® ImageStream®X Mk II (Luminex) is imaging flow cytometer. It is equipped with four lasers for fluorescence imaging (405, 488, 561 and 642 nm), dedicated side scatter (darkfield) laser (785 nm) and possibility of brightfield imaging. Instrument is equiped with two CCD cameras, each with six channels. Lasers 405 and 642 nm are collinear as well as lasers 488 and 561 nm. In total, you can image cells in 12 channels: two are dedicated for brightfield (one on each camera for colocalization purpose); ten channels can be used for fluorescence and side scatter imaging. Imaging cytometer has three objectives (20X, 40X 60X), autofocus, extended depth field functionality and autosampler for 96-well plates.

BC-30 Vet Auto Hematology Analyzer is used for analysing mammal animal blood samples, provides complete blood count, leukocyte differential and haemoglobin concentration measurement results. Instrument measurement is based on impedance of measured cells.

BD Influx (BD Biosciences) is the polychromatic high-speed jet-in-air sorter. It is equipped with five solid state lasers (355, 405, 488, 561 and 640nm). System is equipped with forward (FSC) and side (SSC) scatter detectors and 14 fluorescence detectors, thus it is capable to cover very broad range of fluorochromes and fluorescent proteins. Forward scatter channel has the “small particle option” for the measurement of the particles from 500 nm size.

Optical configuration of the detection path is customizable. System is equipped with automated deposition unit, for sorting into multiwell plates or to other devices. Instrument is capable of six way sorting. Aerosols from the sort chamber are cleared by aerosol evacuation system and whole sorter is enclosed in the class II. biological safety cabinet to maximize the sample protection as well as operator safety.

FACSAria IIu (BD Biosciences) is the high-speed cuvette-based cell sorter. It is equipped with five solid-state lasers (405, 445, 488, 561 and 637nm). System is equipped with forward (FSC) and side (SSC) scatter detectors and with 18 fluorescence detectors.

Optical configuration of the detection path is customizable. System is equipped with automated deposition unit, for sorting into multiwell plates. Instrument is capable of four way sorting.

Automated cell separator AutoMACS Pro (Miltenyi Biotec) utilizes the technology of separation of the target cells, labeled with magnetic nanoparticles, in the strong magnetic field. AutoMACS Pro is capable of automated positive or negative separation of up to 6 cell suspensions in the volumes from 0.2 to 50 ml.

FlowJo is the analysis platform for single-cell flow cytometry analysis with open-ended plugin architecture. The FlowJo workspace is a powerful statistical environment that is used for immunophenotyping, cell cycle, proliferation, kinetics studies, quantitative population comparison, or plate screening assays and more.

Two computers with FlowJo are available in our facility. 30 licenses are distributed among IMG users.

FlowJo workstations: