Project: Impact of mesenchymal cells on the intestinal epithelium homeostasis, regeneration and tumorigenesis
Supervisor
Lucie Janečková
Project description
The intestinal epithelium is a dynamic tissue that provides a protective barrier while supporting nutrient absorption and immune defense of the gut. Stem cells in the crypts renew the epithelium and maintain the intestinal homeostasis, while its dysregulation underlies diseases such as inflammatory bowel disease and colorectal cancer. Intestinal mesenchymal cells play a crucial role in orchestrating these processes by regulating epithelial cell behavior through paracrine signaling, extracellular matrix production, and mechanical interactions. Despite their importance, the mechanisms by which mesenchymal cells influence epithelial cells during intestinal regeneration and in pathological conditions remain poorly understood. Using genetic mouse models, co-culture of intestinal organoids with mesenchymal cells, and methods analyzing gene expression (single cell or bulk RNA sequencing, gene set enrichment analysis), we aim to characterize the role of mesenchymal cells in promoting epithelial renewal in the healthy intestine and their contribution to pathological processes such as tumorigenesis and chronic inflammation. This project offers a unique opportunity to unravel the complex cellular interrelationships in the gut and their implications for health and disease.
Candidate profile
We are seeking a highly motivated and enthusiastic candidate with the following qualifications:
Master’s degree in biological or biomedical sciences, solid background in cell biology and molecular biology.
A strong interest in biomedical research, particularly in the areas of tissue regeneration, inflammation, and cancer biology.
Experience with experimental techniques such as cell culture, microscopy, or molecular biology methods is desirable.
Suggested reading
Brügger MD, Valenta T, Fazilaty H, Hausmann G, Basler K: Distinct populations of crypt-associated fibroblasts act as signaling hubs to control colon homeostasis. PLoS Biol. 2020 18(12):e3001032. [pubmed] [doi]
Kim JE, Fei L, Yin WC, Coquenlorge S, Rao-Bhatia A, Zhang X, Shi SSW, Lee JH, Hahn NA, Rizvi W, Kim KH, Sung HK, Hui CC, Guo G, Kim TH: Single cell and genetic analyses reveal conserved populations and signaling mechanisms of gastrointestinal stromal niches. Nat Commun. 2020 11(1):334. [doi]
