Ústav molekulární genetiky AV ČR, v. v. i.
Join the Holotomography Workshop: Tomocube HT-X1. Theoretical and practical learning experiences from a detailed content lineup.
Registration is required.
Deadline: September 19, 2024
The first half-day will focus on theoretical lectures covering the fundamentals of holotomography using the Tomocube HT-X1 system. Participants will learn about the principles of label-free, 3D refractive index tomography, as well as the system’s unique capabilities in visualizing live cells and tissues in their natural state. The lectures will be focused on principles, technical solutions, and various applications of holotomography, including cell cycle analysis, cell morphology studies, and monitoring dynamic cellular processes. These theoretical sessions will be complemented by user experience presentations.
The remaining two and a half days will be dedicated to hands-on sessions led by an advanced Tomocube applications specialist. These practical sessions will guide participants through system setup, data acquisition, analysis, and export. Attendees are encouraged to bring their own samples for personalized guidance.
This workshop offers a learning experience, combining theoretical knowledge with practical application, under the guidance of an expert.
Tuesday, September 24
| 09:20 – 09:30 | Welcome |
| 09:30 – 10:00 | Denisa Petráčková Non-invasive visualization of bacteria |
| 10:00 – 10:40 | Bruno Combettes Principles of Holotomography and system overview |
| 10:40 – 11:10 | Josef Novak Quantitative imaging of cell migration within ECM Matrigel environment |
| Coffee break | |
| 11:30 – 12:00 | Tomáš Groušl Experience with quantitative imaging and analysis |
| 12:00 – 12:30 | Bruno Combettes Holotomography applications and AI based tools for image quantitative analysis |
| 13:00 | Closing remarks |
| Lunch break | |
| 14:00 – 15:30 | 1st Hands-on session |
Wednesday, September 25
| 09:30 – 11:00 | 2nd Hands-on session |
| 11:15– 12:45 | 3rd Hands-on session |
| Lunch break | |
| 14:00– 15:30 | 4th Hands-on session |
Thursday, September 26
| 09:30 – 11:00 | 5th Hands-on session |
| 11:15– 12:45 | 6th Hands-on session |
| Lunch break | |
| 14:00– 15:30 | 7th Hands-on session |
Friday, September 27
| 09:30 – 13:00 | Open session |
| 13:00 | Closing workshop |
Light Microscopy Core Facility
