| Methods | Fluorescence imaging with lightsheet optical sectioning
Dual-site & multi-field scanning, processing at ZEN Black software
Time-lapse experiments
Multi-positions experiment |
| Illumination | 405nm Solid-state laser, 50mW
445nm Solid-state laser, 25mW
488nm Solid-state laser, 30mW
561nm Solid-state laser, 20mW
638nm Solid-state laser, 75mW
Infrared LED light source for trasmitted light |
| Ilumination objectives | Objective Lightsheet Z.1 5x/0.1
Objective Lightsheet Z.1 10x/0.2 |
| Detection objectives | Dry objective Lightsheet Z.1 5x/0.16
Immersion objective Lightsheet Z.1 10x/0.5
Immersion objective Lightsheet Z.1 20x/1.0
Immersion objective Lightsheet Z.1 40x/1.0
Objective Clr Plan-Apochromat 20x/1.0 Corr nd=1,38
(suited for clearing media with RI 1.38, e.g. Scale)
Objective Clr Plan-Neofluar 20x/1.0 Corr nd=1.45
(suited for clearing media with RI 1.45, e.g. FocusClear) |
| Laser blocking filters | 405/488/561 tripple excitation dichroic
405/488/561/640 quad excitation dichroic
405/488/640 tripple excitation dichroic
445/561 dual excitation dichroic |
| Emission filter sets | DAPI-GFP (SBS LP 490; Em: BP 420-470 + BP 505-545)
DAPI-Cy3 (SBS LP 510; Em: BP 420-470 + BP 575-615)
GFP-Cy3 (SBS LP 560; Em: BP 505-545 + BP 575-615)
GFP-mCherry (SBS LP 560; Em: BP 505-545 + LP 585)
GFP-DRAQ5 (SBS LP 560; Em: BP 505-545 + LP 660)
GFP narrow-mCherry (SBS LP 560; Em: BP 505-530 + LP 585) |
| Cameras | Camera PCO.Edge 5.5 (sCMOS) – 2 pieces; 6,5 µm pixel |
| Additional equipment | Incubation unit (CO2, temperature, humidity)
Antivibration table for SPIM microscopy
PC for storage and data analysis
Stereomicroscope for sample preparation and system maintenance
(Stemi 305 EDU Microscope Set) |
| Software | ZEN Black system 2014 for Lightsheet Z.1 |
| Location | room no. 0.173 (Yellow) |
| Phone | ext. 2471 |
| Booking | Calpendo (“Z1”) |
