Institute of Molecular Genetics of the Czech Academy of Sciences
ScanR high content screening system is built on the inverted widefield microscope Olympus IX83. The system is designed for fully automated image acquisition and data analysis of biological samples. ScanR can handle many different specimen formats, including multi-well plates, slides and custom-built arrays. The hardware autofocus maintains focus stability within the entire specimen. The acquisition is followed by complex image analysis and advanced data evaluation including utilization of pre-trained AI models. Highly sensitive camera enables low fluorescence intensity applications.
| Methods | High-content imaging (widefield) |
| Illumination | Lumencore SPECTRA X IR LED light source for fluorescence (peaks: 377, 438, 475, 510, 555, 575, 635 and 730 nm) Lamp for transmitted light |
| Objectives | UPLSAPO 10x/0.4 DRY; FWD 3.1; CG 0.17 UPLXAPO 20x/0.8 DRY CORR; FWD 0.6; CG 0 – 2 UPLXAPO 40x/0.95 DRY CORR; FWD 0.18; CG 0.11 – 0.23 UPLXAPO 60x/1.42 OIL; FWD 0.15; 0.15 |
| Excitation LED source | UV – DAPI – 377/54 nm (700 mW) Blue – CFP – 438/29 nm (700 mW) Cyan – GFP (FITC) – 475/28 nm (300 mW) Green – TRITC (Cy3) – 555/28 nm (600 mW) Red – Cy5 – 635/22 nm (200 mW) NIR – Cy7 – 730/40 nm (300 mW) |
| Emission filtercubes | PENTA – 5-band dichroic: DAPI/FITC/Cy3/Cy5/Cy7 (409/493/573/652/759 nm) DIC |
| Emission filters | DAPI (432/36) FITC (GFP) (515/30) Cy3 (TRITC) (595/31) Cy5 (685/40) Cy7 (809/81) |
| Cameras | sCMOS camera Hamamatsu ORCA-Fusion BT, 6.5 µm pixel, QE >95%, cooled to -8°C, resolution 2304 x 2304 pixels |
| Stage | Motorized microscope stage xyz |
| Aditional equipment | DFC hardware autofocus |
| Software | ScanR acquisition ScanR analysis |
| Location | room no. 0.175 (Blue) |
| Phone | ext. 2750 |
| Booking | Calpendo (“ScanR-1”) |
ScanR high content screening system is built on the inverted widefield microscope Olympus IX83. The system is designed for fully automated image acquisition and data analysis of biological samples. ScanR can handle many different specimen formats, including multi-well plates, slides and custom-built arrays. The hardware autofocus maintains in-focus stability within the entire specimen. The acquisition is followed by complex image analysis and advanced data evaluation including utilization of pre-trained AI models. Highly sensitive camera enables low fluorescence intensity applications.
| Methods | High-content imaging (widefield) |
| Illumination | Lumencore Spectra X LED light source for fluorescence (peaks: 395, 440, 470, 510, 550, 575 a 640 nm) Lamp for transmitted light |
| Objectives | UPLSAPO 10x/0.4 DRY; FWD 3.1; CG 0.17 UPLXAPO 20x/0.8 DRY CORR; FWD 0.6; CG 0 – 2 UPLXAPO 40x/0.95 DRY CORR; FWD 0.18; CG 0.11 – 0.2 UPLXAPO 60x/1.42 OIL; FWD 0.15; 0.15 |
| Excitation LED source | UV – DAPI 395/25 nm (295mW) Blue – CFP 440/20 nm (256mW) Cyan – FITC 470/24 nm (196mW) Teal – YFP 510/25 nm (52mW) Green – Cy3 550/15 nm (260mW) Red – Cy5 640/30 nm (231mW) |
| Emission filtercubes | D/F/Cy3/Cy5: 4-band dichroic: DAPI/FITC/Cy3/Cy5 (409/493/573/652 nm) CFP/YFP/mCherry: 3-band dichroic: CFP/YFP/mCherry (459/526/596 nm) BF |
| Emission filters | DAPI (433/24) CFP (482/25) FITC (GFP) (520/35) YFP (544/24) Cy3 (TRITC) (600/37) mCherry (641/75) Cy5 (680/42) |
| Cameras | sCMOS camera Hamamatsu ORCA-Fusion BT, 6.5 µm pixel, QE >95%, cooled to -8°C, resolution 2304 x 2304 pixels |
| Stage | Motorized microscope stage xyz |
| Aditional equipment | Hardware autofocus |
| Software | ScanR acquisition ScanR analysis |
| Location | room no. 0.175 (Blue) |
| Phone | ext. 2750 |
| Booking | Calpendo (“ScanR-2”) |