Institute of Molecular Genetics of the Czech Academy of Sciences
The five-day course with theoretical lectures and practical demonstrations and exercises, is intensively devoted to modern methodologies of electron microscopy.
The course is designed for users of electron microscopes in biomedical field, both from scientific laboratories and from core facilities. The aim of the course is to provide an orientation to basic and special techniques in biomedical electron microscopy in an application-oriented manner.
Sample preparation under room temperature and cryo conditions will be covered, including special workflows such as cryo-sectioning, freeze-fracture replica, or cryo-CLEM workflow on FIB-milled lamella. Besides basic imaging, special techniques such as (cryo)FIB-SEM, STEM tomography and EDS analysis will be demonstrated, with introduction to data processing and interpretation.
Special emphasis will be placed on discussing how to choose the appropriate methodology for a specific scientific project.
The course is taught in English.
More information – https://course.img.cas.cz/memls/.