The course theoretically and practically covers methods from high-resolution confocal microscope through computational super-resolution, structured illumination and stimulated depletion microscopy up to the single molecule localization microscopy. During the practical sessions, we will be operating on the scale behind the resolution barrier for standard fluorescence microscope – from lateral resolution 180 nm down to 25 nm.
The course covers the theoretical background and practical experience of all presented super-resolution techniques.
Day 1 | Computation confocal methods – adaptive deconvolution and optical / mathematical reassignment |
Day 2 | Structured illumination methods – from classical SIM to Lattice SIM and DeepSIM |
Day 3 | Nanoscopy – STED and localization microscopy, best practice in deconvolution usage |
More information – https://course.img.cas.cz/slm/.
Looking forward to seeing you in Prague!
Organizing Team