| Methods | Quantitative phase imaging in TL mode
Additional fluorescence imaging
Time-lapse experiments
Multi-position experiments
QPI image advanced analysis |
| Illumination | Lumencore Spectra X – solid-state light source for fluorescence
Transmitted light source halogen lamp EKE 21V |
| Objectives | Nikon PLAN ACHROMAT 4x/0.1 DRY; FWD 30 | BF, QPhase, Hologram, POL, DIC, High Pass Filter (PH)
Nikon PLAN FLUOR 10x/0.3 DRY; FWD 16 | BF, QPhase, Hologram, POL, DIC, High Pass Filter (PH)
Nikon PLAN FLUOR 20x/0.5 DRY; FWD 2.1 | BF, QPhase, Hologram, POL, DIC, High Pass Filter (PH)
Nikon PLAN APO 40x/0.95 DRY, FWD 0.17-0.25 | BF, QPhase, Hologram, POL, DIC, High Pass Filter (PH)
Nikon PLAN APO 60x/1.4 OIL, FWD 0.13| BF, QPhase, Hologram, POL, DIC, High Pass Filter (PH) |
| Filter set | DAPI FITC/Texas Red |
Spectra X excitation
filters
| DAPI 390/22
CFP 434/21
GFP 470/24
FITC 494/20
YFP 511/16
DsRED 555/28
Texas Red 575/25 |
| Dichronic mirrors | QPl-Fluorescence Beam Combiner SP 635 (limits detection of emission wavelengths below 635 nm)
436/514/604 nm tripple band dichroic DAPI/FITC/Texas Red (transmission bands Tavg > 90%: 446-468, 520-540, 614-642)
440/520 nm dual band dichroic CFP/YFP
(transmission bands Tavg > 90%: 449-483, 530-569)
493/574 nm dual band dichroic GFP/DsRed
(transmission bands Tavg > 90%: 500-529, 584-679) |
| Emission filter wheel | DAPI 447/60
CFP 472/30
GFP 514/30
FITC 531/22
YFP 542/27
DsRed 617/73
Texas Red 624/40 |
| Cameras | XIMEA MR4021MC – monochromatic CCD camera for TL; 7,4 µm pixel
Andor Zyla 5.5 – monochromatic sCMOS camera for fluorescence; 6,5 µm pixel |
| Aditional equipment | incubation unit (CO2, temperature, humidity) |
| Software | Q-PHASE software |
| Location | room no. 0.175 (Blue) |
| Phone | ext. 2750 |
| Booking | Calpendo (“QPI”) |
