Confocal Microscopes

Inverted microscope DMi8 with confocal head Leica TCS SP8 is a confocal laser scanning microscope with full transmitted light equipment including transmitted light PMT and Nomarski contrast (DIC) available for all objectives. Leica TCS SP8 it is a dichroic mirror-based system, using interference filters for light filtering and beam-splitting. The confocal head is equipped with two fluorescence PMT detectors and three highly sensitive HyD detectors. The laser power is modulated by acousto-optical tunable filter (AOTF).

The inverted microscope DMi8 with laser scanning confocal head Leica TCS SP8 is a confocal laser scanning microscope with full transmitted light equipment including transmitted light PMT and Nomarski contrast (DIC) available for all objectives. The confocal head is equipped with two fluorescence PMT detectors and three highly sensitive HyD detectors with time resolved gating function useful e.g. for removal of auto-fluorescence. The system has a wide-range white light laser with pulse picker (WLL PP) which allows for very versatile illumination tailored for any fluorophore. The combination of white light laser, AOBS and spectral sliders on detector cascade allows the fine tuning of excitation laser line selection and precise definition of emission spectra. Confocal head light path is equipped with optical image rotation. Stability for long-term live cell imaging experiments is ensured by a hardware autofocus system (AFC) and optional OKOLAB stage-top incubation chamber, which provides temperature, CO₂, and humidity control.

Dragonfly is a fully motorized multi-modal imaging platform with confocal and widefield modes. The core of the microscope is the confocal spinning disk, which combined with high-speed sCMOS and high-sensitive EMCCD cameras provides the system with exceptional speed and sensitivity. The low phototoxicity and photobleaching is ideal for live or delicate specimens. Dragonfly is 10-20 times faster than a traditional laser scanning confocal, which makes it optimal for imaging of fast dynamic events in live cell or for high throughput experiments. Photomanipulation experiments can be performed with Mosaic, a tool that allows for simultaneous illumination of multiple regions of interest of flexible shape and size. The semi-automated microinjector suited for adherent cells microinjection is also available. System is equipped with Super Resolution Radial Fluctuations super-resolution method.

IXplore SpinSR SoRa is a fully motorized multimodal fluorescence microscope designed for gentle and rapid live-cell imaging. Built on the Evident IX83 platform, the system features a modular two-tier frame with an integrated focus adjustment for Köhler illumination and motorized objective turret that can accommodate up to six objectives. The system supports advanced imaging methods, including confocal spinning disk microscopy and super-resolution imaging based on optical reassignment technology. The system integrates cellSense software solution for multi-dimensional data acquisition, including z-stacks, multi-channel, tile-scans, time-lapse, and multi-position imaging. It also features scanR acquisition and analysis software for high-content screening. The microscope is equipped with drift focus correction (DFC) system to maintain z-axis stability during long term experiments.